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美國Cyto Pulse Sciences, Inc. 
美國Cyto Pulse Sciences, Inc.成立于1995年.是一家專業從事電穿孔、電融合儀器研發和生產的公司，多年來致力于研究用于醫療和商業用途的先進脈沖電場技術系統，并將其成果應用于科學研究。擁有獨一無二的 PulseAgile 協議方波、細胞融合和電穿孔專用培養基、控制軟件等多項技術。Cyto Pulse 能夠提供參數(強度、脈寬、方向、重復頻率等)更加快速、靈活變化的脈沖電場，從而實現高效的轉化和融合效率。
　　該公司與全球幾十家科研機構建立了長期合作，其產品接受了廣泛的測試和改進，廣泛的應用于動植物細胞及微生物的轉化、雜交瘤、細胞融合等相關研究。




Cyto Pulse Sciences, Inc. was founded in December 1995 and is incorporated as a regular C corporation in Maryland. The company is composed of a multidisciplinary staff of engineers and scientists. Cyto Pulse develops electric field based devices for use in vaccine delivery, vaccine production, personal vaccine production, gene therapy, and hybridoma production.
In recent years, there have been great advances in immunotherapy and gene therapy against such difficult targets as metastatic cancer, genetic defects, and acute viral infections. In many cases these advances have relied on the use of DNA and RNA. Today there are still no approved clinical uses of this technology because of the inability to deliver, safely and effectively, the DNA/RNA into the target cells. Cyto Pulse, a medical device company, has solved this problem with proprietary technologies using non-viral electric fields. After extensive preclinical studies these are now entering several human trials. The principal Cyto Pulse applications are the production of DNA therapeutic vaccines, delivery of DNA vaccines, modification of cell genomes to correct genetic defects, production of hybridoma cell vaccines by fusing immune system cells and tumor cells, and the production of hybridomas used to produce monoclonal antibodies. The annual markets for these treatments and vaccines are expected to be in the billions of dollars per year. The Cyto Pulse expertise and intellectual property is in waveform generators and electrodes for use in medicine and bioprocessing.

Management

Cyto Pulse Sciences, Inc. was founded by Richard Walters, MSEE, MBA and Alan King, DVM, Ph.D. Mr. Walters worked for the Department of Defense for 13 years, then founded a signal processing company which was eventually acquired by a NASDAQ company. Dr. King retired as a Lt Col. in the Army from the Infectious Diseases Branch at WRAIR. His expertise is in immunology and infectious diseases. Over the years he has spent time conducting infectious disease research in the Thailand, Peru, and Haiti.

Richard E. Walters, MSEE, MBA President and CEO
Alan D. King, DVM, PhD Vice President
John B. Burke, CPA, MBA Secretary and CFO
Derin C. Walters, BSE, MBA (in process) Vice President
Anna-Karin Roos, PhD Manager EU Operations

Facility

Cyto Pulse has a new 7000 square foot facility with a large bio laboratory, R&D electronics laboratory, QSR production facility and 200 square foot ISO 14644 compliant 'Class 5' clean room with sterilization apparatus. Cyto Pulse is located in the Cromwell Industrial Park adjacent to BWI Airport.